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Display of DNA on Nanoparticles for Targeting Antigen Presenting Cells.

ABSTRACT: Efficient delivery of antigens is of paramount concern in immunotherapies. We aimed to target antigen presenting cells (APCs) by conjugating CpG oligonucleotides to an E2 protein nanoparticle surface (CpG-PEG-E2). Compared to E2 alone, we observed ~4-fold increase of in vitro APC uptake of both CpG-PEG-E2 and E2 conjugated to non-CpG DNA. Furthermore, compared to E2-alone or E2 functionalized solely with polyethylene glycol (PEG), the CpG-PEG-E2 showed enhanced lymph node retention up to at least 48 hr and 2-fold increase in APC uptake in vivo, parameters which are advantageous for vaccine success. This suggests that enhanced APC uptake of nanoparticles mediated by oligonucleotide display may help overcome delivery barriers in vaccine development.

SUBMITTER: Molino NM

PROVIDER: S-EPMC5630166 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Display of DNA on Nanoparticles for Targeting Antigen Presenting Cells.

Molino Nicholas M NM Neek Medea M Tucker Jo Anne JA Nelson Edward L EL Wang Szu-Wen SW

ACS biomaterials science & engineering 20170314 4

Efficient delivery of antigens is of paramount concern in immunotherapies. We aimed to target antigen presenting cells (APCs) by conjugating CpG oligonucleotides to an E2 protein nanoparticle surface (CpG-PEG-E2). Compared to E2 alone, we observed ~4-fold increase of <i>in vitro</i> APC uptake of both CpG-PEG-E2 and E2 conjugated to non-CpG DNA. Furthermore, compared to E2-alone or E2 functionalized solely with polyethylene glycol (PEG), the CpG-PEG-E2 showed enhanced lymph node retention up to ...[more]

PMID: 28989957

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