Unknown

Dataset Information

0

Novel chimeric transcript RRM2-c2orf48 promotes metastasis in nasopharyngeal carcinoma.


ABSTRACT: Recently, chimeric transcripts have been found to be associated with the pathogenesis and poor prognosis of malignant tumors. Through our preliminary experiment, a novel chimeric transcript called chimeric transcript RRM2-c2orf48 was detected in C666-1, a classical cell line of human nasopharyngeal carcinoma (NPC). Therefore, the objective of this study was to demonstrate the existence and expression of novel chimeric transcript RRM2-c2orf48 and to explore the main functions and mechanisms of RRM2-c2orf48 in NPC. In this study, the expression of RRM2-c2orf48 was evaluated in NPC cells and specimens. Effects of RRM2-c2orf48 on migration and invasive capacities were detected in vivo and vitro. Moreover, ways in which RRM2-c2orf48 increases the invasive capacities of NPC were explored. As a result, the presence of novel chimeric transcript RRM2-c2orf48 was confirmed in C666-1 by RT-PCR and sequencing, and it was a read-through between RRM2 and c2orf48 through the transcription of interchromosome. Higher expressions of novel RRM2-c2orf48 were detected in NPC cell lines and NPC tissue specimens relative to the controls and its expression was be statistically relevant to TNM staging. High level of RRM2-c2orf48 could increase the migration and invasive capacities of NPC cells, potentially as a result of NPC cell epithelial-mesenchymal transition. RRM2-c2orf48 could also enhance resistance of chemotherapy. In vivo, RRM2-c2orf48 could enhance lung and lymph node metastasis in nude mice. These results demonstrate that high levels of RRM2-c2orf48 expression may be a useful predictor of NPC patients of metastatic potency, presenting potential implications for NPC diagnosis and therapy.

SUBMITTER: Han P 

PROVIDER: S-EPMC5636969 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Novel chimeric transcript RRM2-c2orf48 promotes metastasis in nasopharyngeal carcinoma.

Han Ping P   Chen Ren-Hui RH   Wang Fang F   Zeng Jia-Yi JY   Yu Shi-Tong ST   Xu Li-Hua LH   Cai Qian Q   Liang Fa-Ya FY   Xia Tian-Liang TL   Lin Zhi-Rui ZR   Zhong Qian Q   Huang Xiao-Ming XM  

Cell death & disease 20170914 9


Recently, chimeric transcripts have been found to be associated with the pathogenesis and poor prognosis of malignant tumors. Through our preliminary experiment, a novel chimeric transcript called chimeric transcript RRM2-c2orf48 was detected in C666-1, a classical cell line of human nasopharyngeal carcinoma (NPC). Therefore, the objective of this study was to demonstrate the existence and expression of novel chimeric transcript RRM2-c2orf48 and to explore the main functions and mechanisms of RR  ...[more]

Similar Datasets

2017-06-20 | GSE100193 | GEO
| S-EPMC9035166 | biostudies-literature
| S-EPMC6463592 | biostudies-literature
| S-EPMC7407352 | biostudies-literature
| S-EPMC6156863 | biostudies-literature
| S-EPMC5296651 | biostudies-literature
| S-EPMC3539862 | biostudies-other
| S-EPMC6754338 | biostudies-literature
| S-EPMC6387056 | biostudies-literature
| S-EPMC8758577 | biostudies-literature