Ontology highlight
ABSTRACT:
SUBMITTER: Martinez-Gonzalez CR
PROVIDER: S-EPMC5637266 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Plant methods 20171011
<h4>Background</h4>Genomic DNA extracted from species of Cactaceae is often contaminated with significant amounts of mucilage and pectin. Pectin is one of the main components of cellular walls, whereas mucilage is a complex polysaccharide with a ramified structure. Thus, pectin- and mucilage-free extraction of DNA is a key step for further downstream PCR-based analyses.<h4>Results</h4>We tested our DNA extraction method on cladode tissue (juvenile, adult, and herbaria exemplars) of 17 species of ...[more]