Unknown

Dataset Information

0

Generation and Characterization of Antinonstructural Protein 1 Monoclonal Antibodies and Development of Diagnostics for Dengue Virus Serotype 2.


ABSTRACT: Dengue virus (DENV) circulates in tropical and subtropical areas around the world, where it causes high morbidity and mortality. There is no effective treatment of infection, with supportive care being the only option. Furthermore, early detection and diagnosis are important to facilitate clinical decisions. In this study, seven monoclonal antibodies (mAbs) recognizing nonstructural protein 1 (NS1) of DENV were generated by hybridoma techniques. These antibodies can be divided into two groups: serotype-specific (DB6-1, DB12-3, and DB38-1) and nonspecific (consisting of antibodies DB16-1, DB20-6, DB29-1, and DB41-2). The B-cell epitopes of DB20-6 and DB29-1 were identified by phage display and site-directed mutagenesis, and its binding motif, WXXWGK, was revealed to correspond to amino acid residues 115-120 of the DENV-2 NS1 protein. A diagnostic platform, consisting of a serotype-specific capture antibody and a complex detection antibody, exhibited a detection limit of about 1 ng/mL, which is sufficient to detect NS1 in clinical serum samples from dengue patients. This diagnostic platform displayed better specificity and sensitivity than two examined commercial NS1 diagnostic platforms. In summary, our results indicate that these newly generated mAbs are suitable for detection of NS1 protein of DENV-2 in clinical samples.

SUBMITTER: Tang YL 

PROVIDER: S-EPMC5637597 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Generation and Characterization of Antinonstructural Protein 1 Monoclonal Antibodies and Development of Diagnostics for Dengue Virus Serotype 2.

Tang Yin-Liang YL   Liu I-Ju IJ   Li Pi-Chun PC   Chiu Chien-Yu CY   Lin Chun-Yu CY   Huang Chung-Hao CH   Chen Yen-Hsu YH   Fu Chi-Yu CY   Chao Day-Yu DY   King Chwan-Chuen CC   Wu Han-Chung HC  

The American journal of tropical medicine and hygiene 20170727 4


Dengue virus (DENV) circulates in tropical and subtropical areas around the world, where it causes high morbidity and mortality. There is no effective treatment of infection, with supportive care being the only option. Furthermore, early detection and diagnosis are important to facilitate clinical decisions. In this study, seven monoclonal antibodies (mAbs) recognizing nonstructural protein 1 (NS1) of DENV were generated by hybridoma techniques. These antibodies can be divided into two groups: s  ...[more]

Similar Datasets

| S-EPMC5801815 | biostudies-other
| S-EPMC4239016 | biostudies-literature
| S-EPMC4550467 | biostudies-literature
| S-EPMC4058894 | biostudies-literature
| S-EPMC6698852 | biostudies-literature
| S-EPMC5625772 | biostudies-literature
| S-EPMC4958264 | biostudies-literature
| S-EPMC5997965 | biostudies-literature
| S-EPMC8659457 | biostudies-literature
| S-EPMC3354985 | biostudies-literature