Ontology highlight
ABSTRACT: Background
Xenotransplantation of patient-derived AML (acute myeloid leukemia) cells in NOD-scid Il2r? null (NSG) mice is the method of choice for evaluating this human hematologic malignancy. However, existing models constructed using intravenous injection in adult or newborn NSG mice have inferior engraftment efficiency, poor peripheral blood engraftment, or are difficult to construct.Methods
Here, we describe an improved AML xenograft model where primary human AML cells were injected into NSG newborn pups intrahepatically.Results
Introduction of primary cells from AML patients resulted in high levels of engraftment in peripheral blood, spleen, and bone marrow (BM) of recipient mice. The phenotype of engrafted AML cells remained unaltered during serial transplantation. The mice developed features that are consistent with human AML including spleen enlargement and infiltration of AML cells into multiple organs. Importantly, we demonstrated that although leukemic stem cell activity is enriched and mediated by CD34+CD117+ subpopulation, CD34+CD117- subpopulation can acquire CD34+CD117+ phenotype through de-differentiation. Lastly, we evaluated the therapeutic potential of Sorafenib and Regorafenib in this AML model and found that periphery and spleen AML cells are sensitive to these treatments, whereas BM provides a protective environment to AML.Conclusions
Collectively, our improved model is robust, easy-to-construct, and reliable for pre-clinical AML studies.
SUBMITTER: Her Z
PROVIDER: S-EPMC5639594 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Her Zhisheng Z Yong Kylie Su Mei KSM Paramasivam Kathirvel K Tan Wilson Wei Sheng WWS Chan Xue Ying XY Tan Sue Yee SY Liu Min M Fan Yong Y Linn Yeh Ching YC Hui Kam Man KM Surana Uttam U Chen Qingfeng Q
Journal of hematology & oncology 20171006 1
<h4>Background</h4>Xenotransplantation of patient-derived AML (acute myeloid leukemia) cells in NOD-scid Il2rγ <sup>null</sup> (NSG) mice is the method of choice for evaluating this human hematologic malignancy. However, existing models constructed using intravenous injection in adult or newborn NSG mice have inferior engraftment efficiency, poor peripheral blood engraftment, or are difficult to construct.<h4>Methods</h4>Here, we describe an improved AML xenograft model where primary human AML c ...[more]