Transcriptomic profiles of Clostridium ljungdahlii during lithotrophic growth with syngas or H₂ and CO₂ compared to organotrophic growth with fructose.

ABSTRACT: Clostridium ljungdahlii derives energy by lithotrophic and organotrophic acetogenesis. C. ljungdahlii was grown organotrophically with fructose and also lithotrophically, either with syngas - a gas mixture containing hydrogen (H₂), carbon dioxide (CO₂), and carbon monoxide (CO), or with H₂ and CO₂. Gene expression was compared quantitatively by microarrays using RNA extracted from all three conditions. Gene expression with fructose and with H₂/CO₂ was compared by RNA-Seq. Upregulated genes with both syngas and H₂/CO₂ (compared to fructose) point to the urea cycle, uptake and degradation of peptides and amino acids, response to sulfur starvation, potentially NADPH-producing pathways involving (S)-malate

SUBMITTER: Aklujkar M 

PROVIDER: S-EPMC5640608 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature