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Visualization and quantitation of epidermal growth factor receptor homodimerization and activation with a proximity ligation assay.

ABSTRACT: Activation of the epidermal growth factor receptor (EGFR) results from receptor homodimerization and autophosphorylation and confers sensitivity to tyrosine kinase inhibitors in some tumors. However, the visual detection and quantitation of activated EGFR in the clinical setting has not been established.A proximity ligation assay (PLA) was applied to detect EGFR homodimers in non-small cell lung cancer (NSCLC) cell lines and tissue specimens.PLA signals corresponding to EGFR homodimers were higher in NSCLC cell lines and tissue specimens positive for activating EGFR mutations than in those wild type (WT) for EGFR. Stimulation with EGF in NSCLC cells WT for EGFR or forced overexpression of EGFR in Ba/F3 cells resulted in marked EGFR homodimerization. The extent of EGFR homodimerization appeared related to that of EGFR autophosphorylation in NSCLC cells WT for EGFR.PLA may provide a new tool for detection and quantitation of EGFR homodimers in NSCLC and other tumors.

SUBMITTER: Ota K

PROVIDER: S-EPMC5641116 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Visualization and quantitation of epidermal growth factor receptor homodimerization and activation with a proximity ligation assay.

Ota Keiichi K Harada Taishi T Otsubo Kohei K Fujii Akiko A Tsuchiya Yuko Y Tanaka Kentaro K Okamoto Isamu I Nakanishi Yoichi Y

Oncotarget 20170725 42

<h4>Objectives</h4>Activation of the epidermal growth factor receptor (EGFR) results from receptor homodimerization and autophosphorylation and confers sensitivity to tyrosine kinase inhibitors in some tumors. However, the visual detection and quantitation of activated EGFR in the clinical setting has not been established.<h4>Materials and methods</h4>A proximity ligation assay (PLA) was applied to detect EGFR homodimers in non-small cell lung cancer (NSCLC) cell lines and tissue specimens.<h4>R ...[more]

PMID: 29069773

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Project description:Infectious bronchitis virus (IBV) is a coronavirus responsible for major health problems in the poultry industry. New virus strains continue to appear, causing large economic losses. To develop a rapid and accurate new quantitative assay for diagnosis of the virus without DNA extraction, we selected highly specific single-stranded DNA (ssDNA) aptamers with a high affinity to IBV, using the systematic evolution of ligands by exponential enrichment (SELEX) technology for aptamer screening, followed by high-throughput sequencing technology. Two of these aptamers, AptIBV5 and AptIBV2, were used to establish homogenous and solid-phase proximity ligation assays (PLAs). The developed assays were evaluated for their sensitivity and specificity using collected field samples and then compared to the newly developed sandwich enzyme-linked aptamer assay (ELAA) and reverse transcription-quantitative PCR (qRT-PCR), as the gold-standard method. The solid-phase PLA showed a lower limit of detection and a broader dynamic range than the two other assays. The developed technique may serve as an alternative assay for the diagnosis of IBV, with the potential to be extended to the detection of other important animal or human viruses. IMPORTANCE Infectious bronchitis virus (IBV) causes high morbidity and mortality and large economic losses in the poultry industry. The virus has the ability to genetically mutate into new IBV strains, causing devastating disease and outbreaks. To better monitor the emergence of this virus, the development of a rapid and highly sensitive diagnostic method should be implemented. For this, we generated aptamers with high affinity and specificity to the IBV in an ssDNA library. Using two high-affinity aptamers, we developed a sandwich ELAA and a very sensitive aptamer-based proximity ligation assay (PLA). The new assay showed high sensitivity and specificity and was used to detect IBV in farm samples. The PLA was compared to the newly developed sandwich ELAA and qRT-PCR, as the gold-standard technique.

Dataset Information

Visualization and quantitation of epidermal growth factor receptor homodimerization and activation with a proximity ligation assay.

Publications

Visualization and quantitation of epidermal growth factor receptor homodimerization and activation with a proximity ligation assay.

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