Ontology highlight
ABSTRACT:
SUBMITTER: Schwartz ML
PROVIDER: S-EPMC5643095 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Schwartz Matthew L ML Nickerson Daniel P DP Lobingier Braden T BT Plemel Rachael L RL Duan Mengtong M Angers Cortney G CG Zick Michael M Merz Alexey J AJ
eLife 20170919
Zippering of SNARE complexes spanning docked membranes is essential for most intracellular fusion events. Here, we explore how SNARE regulators operate on discrete zippering states. The formation of a metastable trans-complex, catalyzed by HOPS and its SM subunit Vps33, is followed by subsequent zippering transitions that increase the probability of fusion. Operating independently of Sec18 (NSF) catalysis, Sec17 (α-SNAP) either inhibits or stimulates SNARE-mediated fusion. If HOPS or Vps33 are a ...[more]