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Screening for candidate genes related to breast cancer with cDNA microarray analysis.


ABSTRACT: OBJECTIVE:The aim of this study was to reveal the exact changes during the occurrence of breast cancer to explore significant new and promising genes or factors related to this disease. METHODS:We compared the gene expression profiles of breast cancer tissues with its uninvolved normal breast tissues as controls using the cDNA microarray analysis in seven breast cancer patients. Further, one representative gene, named IFI30, was quantitatively analyzed by real-time PCR to confirm the result of the cDNA microarray analysis. RESULTS:A total of 427 genes were identified with significantly differential expression, 221 genes were up-regulated and 206 genes were down-regulated. And the result of cDNA microarray analysis was validated by detection of IFI30 mRNA level changes by real-time PCR. Genes for cell proliferation, cell cycle, cell division, mitosis, apoptosis, and immune response were enriched in the up-regulated genes, while genes for cell adhesion, proteolysis, and transport were significantly enriched in the down-regulated genes in breast cancer tissues compared with normal breast tissues by a gene ontology analysis. CONCLUSION:Our present study revealed a range of differentially expressed genes between breast cancer tissues and normal breast tissues, and provide candidate genes for further study focusing on the pathogenesis and new biomarkers for breast cancer.

SUBMITTER: Xiang YJ 

PROVIDER: S-EPMC5643563 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Screening for candidate genes related to breast cancer with cDNA microarray analysis.

Xiang Yu-Juan YJ   Fu Qin-Ye QY   Ma Zhong-Bing ZB   Gao De-Zong DZ   Zhang Qiang Q   Li Yu-Yang YY   Li Liang L   Liu Lu L   Ye Chun-Miao CM   Yu Zhi-Gang ZG   Guo Ming-Ming MM  

Chronic diseases and translational medicine 20150305 2


<h4>Objective</h4>The aim of this study was to reveal the exact changes during the occurrence of breast cancer to explore significant new and promising genes or factors related to this disease.<h4>Methods</h4>We compared the gene expression profiles of breast cancer tissues with its uninvolved normal breast tissues as controls using the cDNA microarray analysis in seven breast cancer patients. Further, one representative gene, named IFI30, was quantitatively analyzed by real-time PCR to confirm  ...[more]

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2016-07-01 | GSE70439 | GEO