Ontology highlight
ABSTRACT:
SUBMITTER: Bonanomi M
PROVIDER: S-EPMC5645362 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Bonanomi Marcella M Roffia Valentina V De Palma Antonella A Lombardi Alessio A Aprile Francesco Antonio FA Visentin Cristina C Tortora Paolo P Mauri Pierluigi P Regonesi Maria Elena ME
Scientific reports 20171017 1
The protein ataxin-3 carries a polyglutamine stretch close to the C-terminus that triggers a neurodegenerative disease in humans when its length exceeds a critical threshold. A role as a transcriptional regulator but also as a ubiquitin hydrolase has been proposed for this protein. Here, we report that, when expressed in the yeast Pichia pastoris, full-length ataxin-3 enabled almost normal growth at 37 °C, well above the physiological optimum of 30 °C. The N-terminal Josephin domain (JD) was als ...[more]