Computational design of small transcription activating RNAs for versatile and dynamic gene regulation.

ABSTRACT: A longstanding goal of synthetic biology has been the programmable control of cellular functions. Central to this is the creation of versatile regulatory toolsets that allow for programmable control of gene expression. Of the many regulatory molecules available, RNA regulators offer the intriguing possibility of de novo design-allowing for the bottom-up molecular-level design of genetic control systems. Here we present a computational design approach for the creation of a bacterial regulator called Small Transcription Activating RNAs (STARs) and create a library of high-performing and orthogonal STARs that achieve up to ~ 9000-fold gene activation. We demonstrate the versatility of these STARs-from acting synergistically with existing constitutive and inducible regulators, to reprogramming

SUBMITTER: Chappell J 

PROVIDER: S-EPMC5648800 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature