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Myosin X is recruited to nascent focal adhesions at the leading edge and induces multi-cycle filopodial elongation.


ABSTRACT: Filopodia protrude from the leading edge of cells and play important roles in cell motility. Here we report the mechanism of myosin X (encoded by Myo10)-induced multi-cycle filopodia extension. We found that actin, Arp2/3, vinculin and integrin-? first accumulated at the cell's leading edge. Myosin X was then gathered at these sites, gradually clustered by lateral movement, and subsequently initiated filopodia formation. During filopodia extension, we found the translocation of Arp2/3 and integrin-? along filopodia. Arp2/3 and integrin-? then became localized at the tip of filopodia, from where myosin X initiated the second extension of filopodia with a change in extension direction, thus producing long filopodia. Elimination of integrin-?, Arp2/3 and vinculin by siRNA significantly attenuated the myosin-X-induced long filopodia formation. We propose the following mechanism. Myosin X accumulates at nascent focal adhesions at the cell's leading edge, where myosin X promotes actin convergence to create the base of filopodia. Then myosin X moves to the filopodia tip and attracts integrin-? and Arp2/3 for further actin nucleation. The tip-located myosin X then initiates the second cycle of filopodia elongation to produce the long filopodia.

SUBMITTER: He K 

PROVIDER: S-EPMC5651867 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Myosin X is recruited to nascent focal adhesions at the leading edge and induces multi-cycle filopodial elongation.

He Kangmin K   Sakai Tsuyoshi T   Tsukasaki Yoshikazu Y   Watanabe Tomonobu M TM   Ikebe Mitsuo M  

Scientific reports 20171020 1


Filopodia protrude from the leading edge of cells and play important roles in cell motility. Here we report the mechanism of myosin X (encoded by Myo10)-induced multi-cycle filopodia extension. We found that actin, Arp2/3, vinculin and integrin-β first accumulated at the cell's leading edge. Myosin X was then gathered at these sites, gradually clustered by lateral movement, and subsequently initiated filopodia formation. During filopodia extension, we found the translocation of Arp2/3 and integr  ...[more]

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