Dataset Information

Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes.

ABSTRACT: The acute phase protein orosomucoid-1 (Orm1) is mainly expressed by hepatocytes (HPCs) under stress conditions. However, its specific function is not fully understood. Here, we report a role of Orm1 as an executer of HPC proliferation. Increases in serum levels of Orm1 were observed in patients after surgical resection for liver cancer and in mice undergone partial hepatectomy (PH). Transcriptome study showed that Orm1 became the most abundant in HPCs isolated from regenerating mouse liver tissues after PH. Both in vitro and in vivo siRNA-induced knockdown of Orm1 suppressed proliferation of mouse regenerating HPCs and human hepatic cells. Microarray analysis in regenerating mouse livers revealed that the signaling pathways controlling chromatin replication, especially the minichromosome maintenance protein complex genes were uniformly down-regulated following Orm1 knockdown. These data suggest that Orm1 is induced in response to hepatic injury and executes liver regeneration by activating cell cycle progression in HPCs.

SUBMITTER: Qin XY

PROVIDER: S-EPMC5652006 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes.

Qin Xian-Yang XY Hara Mitsuko M Arner Erik E Kawaguchi Yoshikuni Y Inoue Ikuyo I Tatsukawa Hideki H Furutani Yutaka Y Nagatsuma Keisuke K Matsuura Tomokazu T Wei Feifei F Kikuchi Jun J Sone Hideko H Daub Carsten C Kawaji Hideya H Lassmann Timo T Itoh Masayoshi M Suzuki Harukazu H Carninci Piero P Hayashizaki Yoshihide Y Kokudo Norihiro N Forrest Alistair R R ARR Kojima Soichi S

EBioMedicine 20170912

The acute phase protein orosomucoid-1 (Orm1) is mainly expressed by hepatocytes (HPCs) under stress conditions. However, its specific function is not fully understood. Here, we report a role of Orm1 as an executer of HPC proliferation. Increases in serum levels of Orm1 were observed in patients after surgical resection for liver cancer and in mice undergone partial hepatectomy (PH). Transcriptome study showed that Orm1 became the most abundant in HPCs isolated from regenerating mouse liver tissu ...[more]

PMID: 28927749

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Dataset Information

Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes.

Publications

Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes.

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