Ontology highlight
ABSTRACT:
SUBMITTER: Wing MR
PROVIDER: S-EPMC5652665 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Wing Michele R MR Reeser Julie W JW Smith Amy M AM Reeder Matthew M Martin Dorrelyn D Jewell Benjamin M BM Datta Jharna J Miya Jharna J Monk J Paul JP Mortazavi Amir A Otterson Gregory A GA Goldberg Richard M RM VanDeusen Jeffrey B JB Cole Sharon S Dittmar Kristin K Jaiswal Sunny S Kinzie Matthew M Waikhom Suraj S Freud Aharon G AG Zhou Xiao-Ping XP Chen Wei W Bhatt Darshna D Roychowdhury Sameek S
Oncotarget 20170901 44
Multiplex somatic testing has emerged as a strategy to test patients with advanced cancer. We demonstrate our analytic validation approach for a gene hotspot panel and real-time prospective clinical application for any cancer type. The TruSight Tumor 26 assay amplifies 85 somatic hotspot regions across 26 genes. Using cell line and tumor mixes, we observed that 100% of the 14,715 targeted bases had at least 1000x raw coverage. We determined the sensitivity (100%, 95% CI: 96-100%), positive predi ...[more]