Unknown

Dataset Information

0

Effects of Autologous Platelet-Rich Plasma on Regeneration of Damaged Endometrium in Female Rats.


ABSTRACT: To investigate whether autologous platelet-rich plasma (PRP) treatment can improve regeneration of the endometrium in an experimental model of ethanol-induced damage.Sixty female Sprague-Dawley rats were randomly assigned into three groups: control group, ethanol group, and PRP-treated group (administration of 0.25 mL of PRP into both uterine cavities 72 hours after ethanol injection). After 15 days of endometrial damage, all the animals were sacrificed during the estrous cycle, and samples were taken from the mid-uterine horn. Functional and structural recovery of the endometrium was analyzed by hematoxylin-eosin (H&E) and Masson trichrome (MT) staining, real-time polymerase chain reaction (PCR) assay, and immuno-histochemical (IHC) analyses.H&E and MT staining confirmed significantly decreased fibrosis and increased cellular proliferation in the PRP-treated group, compared to the ethanol group. The endometrial areas in the ethanol and PRP-treated groups were 212.83±15.84 ?m² and 262.34±12.33 ?m² (p=0.065). Significantly stronger IHC expression of cytokeratin, homeobox A10 (HOXA10), vascular endothelial growth factor (VEGF), and Ki-67 was found in the PRP-treated group, compared to the ethanol group. In real-time PCR analyses, interleukin-1? mRNA was down-regulated, while c-Kit mRNA was up-regulated, in the PRP-treated group, compared to the ethanol group.Intrauterine administration of autologous PRP stimulated and accelerated regeneration of the endometrium and also decreased fibrosis in a murine model of damaged endometrium.

SUBMITTER: Jang HY 

PROVIDER: S-EPMC5653485 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Effects of Autologous Platelet-Rich Plasma on Regeneration of Damaged Endometrium in Female Rats.

Jang Hang Yong HY   Myoung Soo Min SM   Choe Jeong Min JM   Kim Tak T   Cheon Yong Pil YP   Kim Yong Min YM   Park Hyuntae H  

Yonsei medical journal 20171101 6


<h4>Purpose</h4>To investigate whether autologous platelet-rich plasma (PRP) treatment can improve regeneration of the endometrium in an experimental model of ethanol-induced damage.<h4>Materials and methods</h4>Sixty female Sprague-Dawley rats were randomly assigned into three groups: control group, ethanol group, and PRP-treated group (administration of 0.25 mL of PRP into both uterine cavities 72 hours after ethanol injection). After 15 days of endometrial damage, all the animals were sacrifi  ...[more]

Similar Datasets

| S-EPMC7086200 | biostudies-literature
| S-EPMC7002419 | biostudies-literature
| S-EPMC5327512 | biostudies-literature
| S-EPMC4828785 | biostudies-literature
| S-EPMC6267832 | biostudies-literature
| S-EPMC6722138 | biostudies-literature
| S-EPMC7472805 | biostudies-literature
| S-EPMC8779764 | biostudies-literature