Biases during DNA extraction affect bacterial and archaeal community profile of anaerobic digestion samples.

ABSTRACT: The anaerobic digestion (AD) of organic waste for biogas production has received much attention in recent years due to the increasing need for renewable energy and environmentally friendly waste management systems. Identification of the microbial community involved in AD aids in better understanding and optimising of the process. The choice of DNA extraction method is an integral step in any molecular biodiversity study. In the present study, potential biases introduced by DNA extraction methods were examined by comparing quality, quantity and representability of DNA extracted from AD samples using various extraction methods. In spite of the non-kit based method (cetyltrimethylammonium bromide) yielding the largest quantity of DNA (approximately 44 µg DNA per gram dry weight), the extracte

SUBMITTER: Roopnarain A 

PROVIDER: S-EPMC5653670 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature