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Virome of US bovine calf serum.

ABSTRACT: Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses. This study further characterizes the diversity of viruses in calf serum with the potential to infect fetuses and through fetal bovine serum contaminate cell cultures.

SUBMITTER: Sadeghi M

PROVIDER: S-EPMC5654489 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Virome of US bovine calf serum.

Sadeghi Mohammadreza M Kapusinszky Beatrix B Yugo Danielle M DM Phan Tung Gia TG Deng Xutao X Kanevsky Isis I Opriessnig Tanja T Woolums Amelia R AR Hurley David J DJ Meng Xiang-Jin XJ Delwart Eric E

Biologicals : journal of the International Association of Biological Standardization 20170115

Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovi ...[more]

PMID: 28100412

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