Ontology highlight
ABSTRACT:
SUBMITTER: Sekhar A
PROVIDER: S-EPMC5654849 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Sekhar Ashok A Nagesh Jayashree J Rosenzweig Rina R Kay Lewis E LE
Protein science : a publication of the Protein Society 20170918 11
The Hsp70 chaperone system plays a critical role in cellular homeostasis by binding to client protein molecules. We have recently shown by methyl-TROSY NMR methods that the Escherichia coli Hsp70, DnaK, can form multiple bound complexes with a small client protein, hTRF1. In an effort to characterize the interactions further we report here the results of an NMR-based titration study of hTRF1 and DnaK, where both molecular components are monitored simultaneously, leading to a binding model. A cen ...[more]