Flow cytometric analysis of CD64 expression pattern and density in the diagnosis of acute promyelocytic leukemia: a multi-center study in Shanghai, China.

ABSTRACT: No unified immunophenotypic profiles and corresponding analytic strategies have been established for the rapid diagnosis of acute promyelocytic leukemia (APL) using flow cytometry (FCM). Here we describe a characteristic immunophenotypic panel that can rapidly and accurately distinguish APL from other types of adult acute myeloid leukemia (AML) using only FCM. By comparing APL cells and non-APL AML cells that share APL common immunophenotypes (CD34^-CD117⁺HLA^-DR^-) we found that CD64 was a significant factor that differentiated APL from other AMLs. Further retrospective analyses of 205 APL and 629 non-APL AML patients from different hematology centers showed that either the CD64^{dim and homo}CD13^+homo CD33^+homoMPO⁺ (myeloperoxidase) CD11c^- panel or the CD64^{dim and homo}CD13^+homo CD33^+homoMPO⁺ CD11c⁺CD10^-CD117⁺ SSC^high (high side scatter signal) panel could distinguish APL from non-APL AML patients with nearly 100% sensitivity, specificity and accuracy. Moreover, relative quantification of CD64 expression enhanced the applicability of our APL diagnostic immunophenotypic panels (ADI-panels) in different hematology centers. Application of the ADI-panels will decrease diagnosis time and improve personalized treatment for APL, a life-threatening disease with very rapid progression.

SUBMITTER: Liu M 

PROVIDER: S-EPMC5655225 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature