Unknown

Dataset Information

0

Coding and noncoding landscape of extracellular RNA released by human glioma stem cells.


ABSTRACT: Tumor-released RNA may mediate intercellular communication and serve as biomarkers. Here we develop a protocol enabling quantitative, minimally biased analysis of extracellular RNAs (exRNAs) associated with microvesicles, exosomes (collectively called EVs), and ribonucleoproteins (RNPs). The exRNA complexes isolated from patient-derived glioma stem-like cultures exhibit distinct compositions, with microvesicles most closely reflecting cellular transcriptome. exRNA is enriched in small ncRNAs, such as miRNAs in exosomes, and precisely processed tRNA and Y RNA fragments in EVs and exRNPs. EV-enclosed mRNAs are mostly fragmented, and UTRs enriched; nevertheless, some full-length mRNAs are present. Overall, there is less than one copy of non-rRNA per EV. Our results suggest that massive EV/exRNA uptake would be required to ensure functional impact of transferred RNA on brain recipient cells and predict the most impactful miRNAs in such conditions. This study also provides a catalog of diverse exRNAs useful for biomarker discovery and validates its feasibility on cerebrospinal fluid.

SUBMITTER: Wei Z 

PROVIDER: S-EPMC5658400 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications


Tumor-released RNA may mediate intercellular communication and serve as biomarkers. Here we develop a protocol enabling quantitative, minimally biased analysis of extracellular RNAs (exRNAs) associated with microvesicles, exosomes (collectively called EVs), and ribonucleoproteins (RNPs). The exRNA complexes isolated from patient-derived glioma stem-like cultures exhibit distinct compositions, with microvesicles most closely reflecting cellular transcriptome. exRNA is enriched in small ncRNAs, su  ...[more]

Similar Datasets

| S-EPMC6195565 | biostudies-literature
2018-01-04 | GSE93143 | GEO
2018-10-30 | GSE113868 | GEO
| S-EPMC8173369 | biostudies-literature
| S-EPMC6735957 | biostudies-literature
| S-EPMC5137021 | biostudies-literature
| S-EPMC8782166 | biostudies-literature
| PRJNA360129 | ENA
| S-EPMC6708067 | biostudies-literature
| S-EPMC6896449 | biostudies-literature