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Larval honey bees infected with Nosema ceranae have increased vitellogenin titers as young adults.

ABSTRACT: Nosema ceranae is a pervasive and widespread honey bee pathogen that is associated with colony declines and has recently been shown to infect larval honey bees. In adult bees, Nosema infection is known to alter levels of a key protein, vitellogenin (Vg), which is necessary for egg-laying in queens, brood food production in workers, and proper immune function in all female bees. We therefore tested the effects of larval worker infection on hemolymph Vg titers. In 1-day old adult workers that were infected as larvae with 10,000 (10?K) or 40,000 (40?K) live N. ceranae spores/bee, Vg titers were significantly elevated by?+?83% and?+?73%, respectively, as compared to controls. At 7 days of adult age, Vg remained significantly elevated (+?68%) in 10?K treated workers as compared to control workers. Nosema infection decreased total hemolymph protein titers in 1 and 7-day old adult bees (-50% in the 10?K and 40?K treated bees). Bees infected as larvae also had a more queen-like sting morphology. They developed slightly but significantly fewer barbs on their stings (-7% in the 40K-treated bees). Higher Vg levels are associated with younger bees. Thus, elevated Vg levels could delay normal age polyethism and disrupt colony balance.

SUBMITTER: BenVau LR

PROVIDER: S-EPMC5658439 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Larval honey bees infected with Nosema ceranae have increased vitellogenin titers as young adults.

BenVau Lee R LR Nieh James C JC

Scientific reports 20171026 1

Nosema ceranae is a pervasive and widespread honey bee pathogen that is associated with colony declines and has recently been shown to infect larval honey bees. In adult bees, Nosema infection is known to alter levels of a key protein, vitellogenin (Vg), which is necessary for egg-laying in queens, brood food production in workers, and proper immune function in all female bees. We therefore tested the effects of larval worker infection on hemolymph Vg titers. In 1-day old adult workers that were ...[more]

PMID: 29075036

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Project description:The microsporidium Nosema ceranae is a high prevalent parasite of the European honey bee (Apis mellifera). This parasite is spreading across the world into its novel host. The developmental process, and some mechanisms of N. ceranae-infected honey bees, has been studied thoroughly; however, few studies have been carried out in the mechanism of gene expression in N. ceranae during the infection process. We therefore performed the suppressive subtractive hybridization (SSH) approach to investigate the candidate genes of N. ceranae during its infection process. All 96 clones of infected (forward) and non-infected (reverse) library were dipped onto the membrane for hybridization. A total of 112 differentially expressed sequence tags (ESTs) had been sequenced. For the host responses, 20% of ESTs (13 ESTs, 10 genes, and 1 non-coding RNA) from the forward library and 93.6% of ESTs (44 ESTs, 28 genes) from the reverse library were identified as differentially expressed genes (DEGs) of the hosts. A high percentage of DEGs involved in catalytic activity and metabolic processes revealed that the host gene expression change after N. ceranae infection might lead to an unbalance of physiological mechanism. Among the ESTs from the forward library, 75.4% ESTs (49 ESTs belonged to 24 genes) were identified as N. ceranae genes. Out of 24 N. ceranae genes, nine DEGs were subject to real-time quantitative reverse transcription PCR (real-time qRT-PCR) for validation. The results indicated that these genes were highly expressed during N. ceranae infection. Among nine N. ceranae genes, one N. ceranae gene (AAJ76_1600052943) showed the highest expression level after infection. These identified differentially expressed genes from this SSH could provide information about the pathological effects of N. ceranae. Validation of nine up-regulated N. ceranae genes reveal high potential for the detection of early nosemosis in the field and provide insight for further applications.

Dataset Information

Larval honey bees infected with Nosema ceranae have increased vitellogenin titers as young adults.

Publications

Larval honey bees infected with Nosema ceranae have increased vitellogenin titers as young adults.

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