Ca²⁺ protein alpha 1D of CaV1.3 regulates intracellular calcium concentration and migration of colon cancer cells through a non-canonical activity.

ABSTRACT: It is generally accepted that voltage-gated Ca²⁺ channels, CaV, regulate Ca²⁺ homeostasis in excitable cells following plasma membrane depolarization. Here, we show that the Ca²⁺ protein α1D of CaV1.3 channel is overexpressed in colorectal cancer biopsies compared to normal tissues. Gene silencing experiments targeting α1D reduced the migration and the basal cytosolic Ca²⁺ concentration of HCT116 colon cancer cell line and modified the cytosolic Ca²⁺ oscillations induced by the sodium/calcium exchanger NCX1/3 working in its reverse mode. Interestingly, NCX1/3 regulated membrane potential of HCT116 cells only when α1D was silenced, and blocking NCX1/3 increased cytosolic Ca²⁺ concentration and cell migration. However, membran

SUBMITTER: Fourbon Y 

PROVIDER: S-EPMC5660277 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature