A Biomimetic Phosphatidylcholine-Terminated Monolayer Greatly Improves the In Vivo Performance of Electrochemical Aptamer-Based Sensors.

ABSTRACT: The real-time monitoring of specific analytes in?situ in the living body would greatly advance our understanding of physiology and the development of personalized medicine. Because they are continuous (wash-free and reagentless) and are able to work in complex media (e.g., undiluted serum), electrochemical aptamer-based (E-AB) sensors are promising candidates to fill this role. E-AB sensors suffer, however, from often-severe baseline drift when deployed in undiluted whole blood either in?vitro or in?vivo. We demonstrate that cell-membrane-mimicking phosphatidylcholine (PC)-terminated monolayers improve the performance of E-AB sensors, reducing the baseline drift from around 70?% to just a few percent after several hours in flowing whole blood in?vitro. With this improvement comes the ability to deploy E-AB sensors directly in?situ in the veins of live animals, achieving micromolar precision over many hours without the use of physical barriers or active drift-correction algorithms.

SUBMITTER: Li H 

PROVIDER: S-EPMC5660315 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature