Ontology highlight
ABSTRACT:
SUBMITTER: De Tullio L
PROVIDER: S-EPMC5663194 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
De Tullio Luisina L Kaniecki Kyle K Kwon Youngho Y Crickard J Brooks JB Sung Patrick P Greene Eric C EC
Cell reports 20171001 3
Srs2 is a super-family 1 helicase that promotes genome stability by dismantling toxic DNA recombination intermediates. However, the mechanisms by which Srs2 remodels or resolves recombination intermediates remain poorly understood. Here, single-molecule imaging is used to visualize Srs2 in real time as it acts on single-stranded DNA (ssDNA) bound by protein factors that function in recombination. We demonstrate that Srs2 is highly processive and translocates rapidly (∼170 nt per second) in the 3 ...[more]