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The Streptococcus mutans irvA gene encodes a trans-acting riboregulatory mRNA.


ABSTRACT: In both prokaryotes and eukaryotes, insight into gene function is typically obtained by in silico homology searches and/or phenotypic analyses of strains bearing mutations within open reading frames. However, the studies herein illustrate how mRNA function is not limited to the expression of a cognate protein. We demonstrate that a stress-induced protein-encoding mRNA (irvA) from the dental caries pathogen Streptococcus mutans directly modulates target mRNA (gbpC) stability through seed pairing interactions. The 5' untranslated region of irvA mRNA is a trans riboregulator of gbpC and a critical activator of the DDAG stress response, whereas IrvA functions independently in the regulation of natural competence. The irvA riboregulatory domain controls GbpC production by forming irvA-gbpC hybrid mRNA duplexes that prevent gbpC degradation by an RNase J2-mediated pathway. These studies implicate a potentially ubiquitous role for typical protein-encoding mRNAs as riboregulators, which could alter current concepts in gene regulation.

SUBMITTER: Liu N 

PROVIDER: S-EPMC5663275 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

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The Streptococcus mutans irvA gene encodes a trans-acting riboregulatory mRNA.

Liu Nan N   Niu Guoqing G   Xie Zhoujie Z   Chen Zhiyun Z   Itzek Andreas A   Kreth Jens J   Gillaspy Allison A   Zeng Lin L   Burne Robert R   Qi Fengxia F   Merritt Justin J  

Molecular cell 20150101 1


In both prokaryotes and eukaryotes, insight into gene function is typically obtained by in silico homology searches and/or phenotypic analyses of strains bearing mutations within open reading frames. However, the studies herein illustrate how mRNA function is not limited to the expression of a cognate protein. We demonstrate that a stress-induced protein-encoding mRNA (irvA) from the dental caries pathogen Streptococcus mutans directly modulates target mRNA (gbpC) stability through seed pairing  ...[more]

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