Designing broad-spectrum anti-HIV-1 gRNAs to target patient-derived variants.
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ABSTRACT:
SUBMITTER: Dampier W
PROVIDER: S-EPMC5663707 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Publications
Designing broad-spectrum anti-HIV-1 gRNAs to target patient-derived variants.
Dampier Will W Sullivan Neil T NT Chung Cheng-Han CH Mell Joshua Chang JC Nonnemacher Michael R MR Wigdahl Brian B
Scientific reports 20171031 1
Clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated protein 9 (Cas9), including specific guide RNAs (gRNAs), can excise integrated human immunodeficiency virus type 1 (HIV-1) provirus from host chromosomes. To date, anti-HIV-1 gRNAs have been designed to account for off-target activity, however, they seldom account for genetic variation in the HIV-1 genome within and between patients, which will be crucial for therapeutic application of this technology. This anal ...[more]
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