Unknown

Dataset Information

0

Genome-wide profiling of nardilysin target genes reveals its role in epigenetic regulation and cell cycle progression.


ABSTRACT: Post-translational histone modifications, such as acetylation and methylation, are prerequisites for transcriptional regulation. The metalloendopeptidase nardilysin (Nrdc) is a H3K4me2-binding protein that controls thermoregulation and ?-cell functions through its transcriptional coregulator function. We herein combined high-throughput ChIP-seq and RNA-seq to achieve the first genome-wide identification of Nrdc target genes. A ChIP-seq analysis of immortalized mouse embryo fibroblasts (iMEF) identified 4053 Nrdc-binding sites, most of which were located in proximal promoter sites (2587 Nrdc-binding genes). Global H3K4me2 levels at Nrdc-binding promoters slightly increased, while H3K9ac levels decreased in the absence of Nrdc. Among Nrdc-binding genes, a comparative RNA-seq analysis identified 448 candidates for Nrdc target genes, among which cell cycle-related genes were significantly enriched. We confirmed decreased mRNA and H3K9ac levels at the promoters of individual genes in Nrdc-deficient iMEF, which were restored by the ectopic introduction of Nrdc. Reduced mRNA levels, but not H3K9ac levels were fully restored by the reintroduction of the peptidase-dead mutant of Nrdc. Furthermore, Nrdc promoted cell cycle progression at multiple stages, which enhanced cell proliferation in vivo. Collectively, our integrative studies emphasize the importance of Nrdc for maintaining a proper epigenetic status and cell growth.

SUBMITTER: Morita Y 

PROVIDER: S-EPMC5665917 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Genome-wide profiling of nardilysin target genes reveals its role in epigenetic regulation and cell cycle progression.

Morita Yusuke Y   Ohno Mikiko M   Nishi Kiyoto K   Hiraoka Yoshinori Y   Saijo Sayaka S   Matsuda Shintaro S   Kita Toru T   Kimura Takeshi T   Nishi Eiichiro E  

Scientific reports 20171101 1


Post-translational histone modifications, such as acetylation and methylation, are prerequisites for transcriptional regulation. The metalloendopeptidase nardilysin (Nrdc) is a H3K4me2-binding protein that controls thermoregulation and β-cell functions through its transcriptional coregulator function. We herein combined high-throughput ChIP-seq and RNA-seq to achieve the first genome-wide identification of Nrdc target genes. A ChIP-seq analysis of immortalized mouse embryo fibroblasts (iMEF) ide  ...[more]

Similar Datasets

| S-EPMC9260311 | biostudies-literature
| S-EPMC3657391 | biostudies-literature
| S-EPMC6511647 | biostudies-literature
| S-EPMC6981219 | biostudies-literature
| S-EPMC6991687 | biostudies-literature
| S-EPMC4845196 | biostudies-other
| S-EPMC4542010 | biostudies-literature
| S-EPMC5778740 | biostudies-literature
| S-EPMC5701423 | biostudies-literature
| S-EPMC4667427 | biostudies-literature