Unknown

Dataset Information

0

Clenbuterol Induces Cell Cycle Arrest in C2C12 Myoblasts by Delaying p27 Degradation through ?-arrestin 2 Signaling.

ABSTRACT: ?2-Adrenoceptor (?2-AR) agonists promote muscle growth. The aim of this study was to elucidate some effects of the selective ?2-adrenoceptor agonist clenbuterol (CLB) on myoblast proliferation. We found that CLB induces cell cycle arrest in C2C12 myoblasts. This effect is partly due to the enhanced stability of p27, rather than the increased gene transcription via cAMP response element-binding protein (CREB). Specifically, CLB treatment enhanced the accumulation of p27 in the nucleus while depleting it from the cytosol via a mechanism that requires ?2-AR. Surprisingly, p27 accumulation was not reversed by the protein kinase A (PKA) inhibitor H-89, but interestingly, was alleviated by the knockdown of ?-arrestin 2. Thus, our work provides a basis for ?2-AR agonists inhibit myoblasts proliferation through signaling via ?2-AR, ?-arrestin 2, and p27.

SUBMITTER: Chen M 

PROVIDER: S-EPMC5666532 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Clenbuterol Induces Cell Cycle Arrest in C2C12 Myoblasts by Delaying p27 Degradation through β-arrestin 2 Signaling.

Chen Min M   Liu Chuncheng C   Wang Meng M   Wang Hong H   Zhang Kuo K   Zheng Yu Y   Yu Zhengquan Z   Li Xiangdong X   Guo Wei W   Li Ning N   Meng Qingyong Q  

International journal of biological sciences 20171017 10

β<sub>2</sub>-Adrenoceptor (β<sub>2</sub>-AR) agonists promote muscle growth. The aim of this study was to elucidate some effects of the selective β<sub>2</sub>-adrenoceptor agonist clenbuterol (CLB) on myoblast proliferation. We found that CLB induces cell cycle arrest in C2C12 myoblasts. This effect is partly due to the enhanced stability of p27, rather than the increased gene transcription via cAMP response element-binding protein (CREB). Specifically, CLB treatment enhanced the accumulation  ...[more]

Similar Datasets

Unknown Vitamin C Activates the Folate-Mediated One-Carbon Cycle in C2C12 Myoblasts.
| S-EPMC7139526 | biostudies-literature
Unknown Kinetin stimulates differentiation of C2C12 myoblasts.
| S-EPMC8513909 | biostudies-literature
Unknown Metabolic effects of carvedilol through β-arrestin proteins: investigations in a streptozotocin-induced diabetes rat model and in C2C12 myoblasts.
| S-EPMC7707093 | biostudies-literature
Unknown Clathrin adaptor GGA1 modulates myogenesis of C2C12 myoblasts.
| S-EPMC6237421 | biostudies-literature
Unknown Targeting the p27 E3 ligase SCF(Skp2) results in p27- and Skp2-mediated cell-cycle arrest and activation of autophagy.
| S-EPMC2343599 | biostudies-literature
Unknown The p27-Skp2 axis mediates glucocorticoid-induced cell cycle arrest in T-lymphoma cells.
| S-EPMC3865052 | biostudies-literature
Unknown Myc inhibits p27-induced erythroid differentiation of leukemia cells by repressing erythroid master genes without reversing p27-mediated cell cycle arrest.
| S-EPMC2593424 | biostudies-literature
Unknown Identification of PRDM2 regulated genes in quiescent C2C12 myoblasts.
| S-EPMC4664777 | biostudies-literature
Unknown Molecular Toxicological Mechanisms of Synthetic Cathinones on C2C12 Myoblasts.
| S-EPMC6479684 | biostudies-literature
Unknown The vitamin D receptor regulates mitochondrial function in C2C12 myoblasts.
| S-EPMC7099523 | biostudies-literature