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How to split a G-quadruplex for DNA detection: new insight into the formation of DNA split G-quadruplex.

ABSTRACT: Here, we get a new insight into the formation of a split G-quadruplex from the viewpoints of the split mode and guanine base number. An unusual result is that the split mode 4?:?8 performed best in six split modes, including the frequently used mode 1?:?3 and 2?:?2 in the split G-quadruplex enhanced fluorescence assay. Circular dichroism spectra verified the conclusion. The application of the split G-quadruplex based assay in DNA detection was performed on the point mutations of the JAK2 V617F and HBB genes. A multi-target analysis method based on a pool of G-segments split from T30695 (GGGTGGGTGGGTGGGT) by the magic "law of 4?:?8" was established.

SUBMITTER: Zhu J

PROVIDER: S-EPMC5667574 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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How to split a G-quadruplex for DNA detection: new insight into the formation of DNA split G-quadruplex.

Zhu Jinbo J Zhang Libing L Dong Shaojun S Wang Erkang E

Chemical science 20150602 8

Here, we get a new insight into the formation of a split G-quadruplex from the viewpoints of the split mode and guanine base number. An unusual result is that the split mode 4 : 8 performed best in six split modes, including the frequently used mode 1 : 3 and 2 : 2 in the split G-quadruplex enhanced fluorescence assay. Circular dichroism spectra verified the conclusion. The application of the split G-quadruplex based assay in DNA detection was performed on the point mutations of the JAK2 V617F a ...[more]

PMID: 29142717

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