Ontology highlight
ABSTRACT:
SUBMITTER: Azofeifa JG
PROVIDER: S-EPMC5667649 | biostudies-literature | 2017 Sep-Oct
REPOSITORIES: biostudies-literature
Azofeifa Joseph G JG Allen Mary A MA Lladser Manuel E ME Dowell Robin D RD
IEEE/ACM transactions on computational biology and bioinformatics 20160126 5
We present a fast and simple algorithm to detect nascent RNA transcription in global nuclear run-on sequencing (GRO-seq). GRO-seq is a relatively new protocol that captures nascent transcripts from actively engaged polymerase, providing a direct read-out on bona fide transcription. Most traditional assays, such as RNA-seq, measure steady state RNA levels which are affected by transcription, post-transcriptional processing, and RNA stability. GRO-seq data, however, presents unique analysis challe ...[more]