Dataset Information

Character-based DNA barcoding for authentication and conservation of IUCN Red listed threatened species of genus Decalepis (Apocynaceae).

ABSTRACT: The steno-endemic species of genus Decalepis are highly threatened by destructive wild harvesting. The medicinally important fleshy tuberous roots of Decalepis hamiltonii are traded as substitute, to meet the international market demand of Hemidesmus indicus. In addition, the tuberous roots of all three species of Decalepis possess similar exudates and texture, which challenges the ability of conventional techniques alone to perform accurate species authentication. This study was undertaken to generate DNA barcodes that could be utilized in monitoring and curtailing the illegal trade of these endangered species. The DNA barcode reference library was developed in BOLD database platform for candidate barcodes rbcL, matK, psbA-trnH, ITS and ITS2. The average intra-specific variations (0-0.27%) were less than the distance to nearest neighbour (0.4-11.67%) with matK and ITS. Anchoring the coding region rbcL in multigene tiered approach, the combination rbcL?+?matK?+?ITS yielded 100% species resolution, using the least number of loci combinations either with PAUP or BLOG methods to support a character-based approach. Species-specific SNP position (230?bp) in the matK region that is characteristic of D. hamiltonii could be used to design specific assays, enhancing its applicability for direct use in CITES enforcement for distinguishing it from H. indicus.

SUBMITTER: Mishra P

PROVIDER: S-EPMC5668324 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Character-based DNA barcoding for authentication and conservation of IUCN Red listed threatened species of genus Decalepis (Apocynaceae).

Mishra Priyanka P Kumar Amit A Sivaraman Gokul G Shukla Ashutosh K AK Kaliamoorthy Ravikumar R Slater Adrian A Velusamy Sundaresan S

Scientific reports 20171102 1

The steno-endemic species of genus Decalepis are highly threatened by destructive wild harvesting. The medicinally important fleshy tuberous roots of Decalepis hamiltonii are traded as substitute, to meet the international market demand of Hemidesmus indicus. In addition, the tuberous roots of all three species of Decalepis possess similar exudates and texture, which challenges the ability of conventional techniques alone to perform accurate species authentication. This study was undertaken to g ...[more]

PMID: 29097709

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Project description:The biodiversity crisis we live in, marked by high extinction rates, requires well-planned conservation efforts. To overcome this issue, red lists of threatened species are recognized as the main objective approach for evaluating the conservation status of species and therefore guiding conservation priorities. This work focuses on the Myriapoda (Chilopoda and Diplopoda) species listed in the Brazilian red list of fauna to enable discussion of the practical implications of red lists for conservation. Almost all myriapods assessed are endemic to Brazil (99 %) and 73 % are known from subterranean habitats only. Despite of 33 % being recorded from protected areas (PAs), downgrading, degazettement or downsizing of PAs and intense and unregulated ecotourism represent great threats. The PAs network in Brazil tends to fail in conserving myriapod species. The number of data deficient species (42 %) states the need of investing in ecological and taxonomic studies about the group, in order to fill in important knowledge gaps in species assessments nationally and globally. In this work we show that there is a lack of communication between national and global agencies concerning red lists, which results in a significant loss for science and for conservation. Despite investing in national and state red lists, individual countries must take the final step of submitting its data to IUCN global database, as significant international funding is available for IUCN red listed species conservation. Being one of the most diverse countries in the world, and facing the biggest cuts ever on national science funding, losing these important funding opportunities is a huge loss for Brazilian biodiversity conservation and for science. This study raises awareness on subterranean habitats conservation, due to its high endemism and fragility. Since the first edition of the Brazilian Red List in 1968, centipedes are now included for the first time, and millipedes for the second time. The presence of these myriapods in the list brings attention to the group, which usually receives little or no attention in conservation programs and environmental impact assessments. Rather than a specific case for Myriapoda and for Brazil, the points discussed here can be related to arthropods and the tropics, as the most biodiverse countries are emerging economies facing similar challenges in PAs network management, species extinction risks and science funding.

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Dataset Information

Character-based DNA barcoding for authentication and conservation of IUCN Red listed threatened species of genus Decalepis (Apocynaceae).

Publications

Character-based DNA barcoding for authentication and conservation of IUCN Red listed threatened species of genus Decalepis (Apocynaceae).

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