Unknown

Dataset Information

0

Salbutamol inhibits ubiquitin-mediated survival motor neuron protein degradation in spinal muscular atrophy cells.


ABSTRACT: Spinal muscular atrophy (SMA) is a common autosomal recessive neuromuscular disorder that is currently incurable. SMA is caused by decreased levels of the survival motor neuron protein (SMN), as a result of loss or mutation of SMN1. Although the SMN1 homolog SMN2 also produces some SMN protein, it does not fully compensate for the loss or dysfunction of SMN1. Salbutamol, a ?2-adrenergic receptor agonist and well-known bronchodilator used in asthma patients, has recently been shown to ameliorate symptoms in SMA patients. However, the precise mechanism of salbutamol action is unclear. We treated SMA fibroblast cells lacking SMN1 and HeLa cells with salbutamol and analyzed SMN2 mRNA and SMN protein levels in SMA fibroblasts, and changes in SMN protein ubiquitination in HeLa cells. Salbutamol increased SMN protein levels in a dose-dependent manner in SMA fibroblast cells lacking SMN1, though no significant changes in SMN2 mRNA levels were observed. Notably, the salbutamol-induced increase in SMN was blocked by a protein kinase A (PKA) inhibitor and deubiquitinase inhibitor, respectively. Co-immunoprecipitation assay using HeLa cells showed that ubiquitinated SMN levels decreased in the presence of salbutamol, suggesting that salbutamol inhibited ubiquitination. The results of this study suggest that salbutamol may increase SMN protein levels in SMA by inhibiting ubiquitin-mediated SMN degradation via activating ?2-adrenergic receptor-PKA pathways.

SUBMITTER: Harahap NIF 

PROVIDER: S-EPMC5669398 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Salbutamol inhibits ubiquitin-mediated survival motor neuron protein degradation in spinal muscular atrophy cells.

Harahap Nur Imma Fatimah NIF   Nurputra Dian Kesumapramudya DK   Ar Rochmah Mawaddah M   Shima Ai A   Morisada Naoya N   Takarada Toru T   Takeuchi Atsuko A   Tohyama Yumi Y   Yanagisawa Shinichiro S   Nishio Hisahide H  

Biochemistry and biophysics reports 20151028


Spinal muscular atrophy (SMA) is a common autosomal recessive neuromuscular disorder that is currently incurable. SMA is caused by decreased levels of the survival motor neuron protein (SMN), as a result of loss or mutation of <i>SMN1</i>. Although the <i>SMN1</i> homolog <i>SMN2</i> also produces some SMN protein, it does not fully compensate for the loss or dysfunction of <i>SMN1</i>. Salbutamol, a β2-adrenergic receptor agonist and well-known bronchodilator used in asthma patients, has recent  ...[more]

Similar Datasets

| S-EPMC3527959 | biostudies-literature
| S-EPMC3696827 | biostudies-other
| S-EPMC5179954 | biostudies-literature
| S-EPMC2853768 | biostudies-literature
| S-EPMC3164180 | biostudies-literature
| S-EPMC4090017 | biostudies-literature
| S-EPMC7496476 | biostudies-literature
| S-EPMC9813745 | biostudies-literature
2017-04-10 | GSE86908 | GEO
| S-EPMC3474434 | biostudies-literature