Required hydrophobicity of fluorescent reporters for phosphatidylinositol family of lipid enzymes.

ABSTRACT: The phosphatidylinositol (PtdIns) family of lipids plays important roles in cell differentiation, proliferation, and migration. Abnormal expression, mutation, or regulation of their metabolic enzymes has been associated with various human diseases such as cancer, diabetes, and bipolar disorder. Recently, fluorescent derivatives have increasingly been used as chemical probes to monitor either lipid localization or enzymatic activity. However, the requirements of a good probe have not been well defined, particularly modifications on the diacylglycerol side chain partly due to challenges in generating PtdIns lipids. We have synthesized a series of fluorescent PtdIns(4,5)P₂ (PIP₂) and PtdIns (PI) derivatives with various lengths of side chains and tested their capacity as

SUBMITTER: Waybright J 

PROVIDER: S-EPMC5671358 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature