Phosphoproteomic Analysis Reveals the Importance of Kinase Regulation During Orbivirus Infection.

ABSTRACT: Bluetongue virus (BTV) causes infections in wild and domesticated ruminants with high morbidity and mortality and is responsible for significant economic losses in both developing and developed countries. BTV serves as a model for the study of other members of the Orbivirus genus. Previously, the importance of casein kinase 2 for BTV replication was demonstrated. To identify intracellular signaling pathways and novel host-cell kinases involved during BTV infection, the phosphoproteome of BTV infected cells was analyzed. Over 1000 phosphosites were identified using mass spectrometry, which were then used to determine the corresponding kinases involved during BTV infection. This analysis yielded protein kinase A (PKA) as a novel kinase activated during BTV infection. Subsequently, the

SUBMITTER: Mohl BP 

PROVIDER: S-EPMC5672004 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature