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Effects of iron nanoparticles on iron-corroding bacteria.

ABSTRACT: The toxicological effects of Fe3O4 nanoparticles were evaluated with an iron-corroding bacterium (ICB) for preventing the biocorrosion of iron. Fe3O4 nanoparticles of 18 nm were successfully prepared and characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) patterns and scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS). A halophilic ICB strain L4 was isolated from Ribandar saltpan Goa, India and identified biochemically and by 16S rRNA gene sequence analysis as Halanaerobium sp. The Fe3O4 nanoparticles in increasing doses (0.1-100 mg/L) caused transformation in growth and sulfide production of ICB strain L4. SEM-EDS analysis revealed a deformed cell structure with adsorption of nanoparticle on the cell surface and increased cell size. Comet assay revealed genotoxic effect of Fe3O4 nanoparticles on strain L4 which resulted in dose-dependent DNA damage by increasing percentage tail DNA from 5 to 88% with increasing Fe3O4 nanoparticles concentration. Furthermore, sulfide production rate was reduced to 11.8% in presence of 100 mg/L Fe3O4 nanoparticles which reduced the corroding property of ICB strain L4; thus, it was unable to corrode the iron nail in presence of Fe3O4 nanoparticle. This work suggests the possible application of Fe3O4 nanoparticle in addressing biocorrosion problems faced by different industries.

SUBMITTER: Das KR

PROVIDER: S-EPMC5673863 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Effects of iron nanoparticles on iron-corroding bacteria.

Das Kirti Ranjan KR Kerkar Savita S Meena Yogeeta Y Mishra Samir S

3 Biotech 20171106 6

The toxicological effects of Fe3O4 nanoparticles were evaluated with an iron-corroding bacterium (ICB) for preventing the biocorrosion of iron. Fe3O4 nanoparticles of 18 nm were successfully prepared and characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) patterns and scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS). A halophilic ICB strain L4 was isolated from Ribandar saltpan Goa, India and iden ...[more]

PMID: 29201585

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Project description:Microbially induced concrete corrosion (MICC) in sewer systems has been a serious problem for a long time. A better understanding of the succession of microbial community members responsible for the production of sulfuric acid is essential for the efficient control of MICC. In this study, the succession of sulfur-oxidizing bacteria (SOB) in the bacterial community on corroding concrete in a sewer system in situ was investigated over 1 year by culture-independent 16S rRNA gene-based molecular techniques. Results revealed that at least six phylotypes of SOB species were involved in the MICC process, and the predominant SOB species shifted in the following order: Thiothrix sp., Thiobacillus plumbophilus, Thiomonas intermedia, Halothiobacillus neapolitanus, Acidiphilium acidophilum, and Acidithiobacillus thiooxidans. A. thiooxidans, a hyperacidophilic SOB, was the most dominant (accounting for 70% of EUB338-mixed probe-hybridized cells) in the heavily corroded concrete after 1 year. This succession of SOB species could be dependent on the pH of the concrete surface as well as on trophic properties (e.g., autotrophic or mixotrophic) and on the ability of the SOB to utilize different sulfur compounds (e.g., H2S, S0, and S2O3(2-)). In addition, diverse heterotrophic bacterial species (e.g., halo-tolerant, neutrophilic, and acidophilic bacteria) were associated with these SOB. The microbial succession of these microorganisms was involved in the colonization of the concrete and the production of sulfuric acid. Furthermore, the vertical distribution of microbial community members revealed that A. thiooxidans was the most dominant throughout the heavily corroded concrete (gypsum) layer and that A. thiooxidans was most abundant at the highest surface (1.5-mm) layer and decreased logarithmically with depth because of oxygen and H2S transport limitations. This suggested that the production of sulfuric acid by A. thiooxidans occurred mainly on the concrete surface and the sulfuric acid produced penetrated through the corroded concrete layer and reacted with the sound concrete below.

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Effects of iron nanoparticles on iron-corroding bacteria.

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Effects of iron nanoparticles on iron-corroding bacteria.

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