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Mutation accumulation under UV radiation in Escherichia coli.

ABSTRACT: Mutations are induced by not only intrinsic factors such as inherent molecular errors but also by extrinsic mutagenic factors such as UV radiation. Therefore, identifying the mutational properties for both factors is necessary to achieve a comprehensive understanding of evolutionary processes both in nature and in artificial situations. Although there have been extensive studies on intrinsic factors, the mutational profiles of extrinsic factors are poorly understood on a genomic scale. Here, we explored the mutation profiles of UV radiation, a ubiquitous mutagen, in Escherichia coli on the genomic scale. We performed an evolution experiment under periodic UV radiation for 28 days. The accumulation speed of the mutations was found to increase so that it exceeded that of a typical mutator strain with deficient mismatch repair processes. The huge contribution of the extrinsic factors to all mutations consequently increased the risk of the destruction of inherent error correction systems. The spectrum of the UV-induced mutations was broader than that of the spontaneous mutations in the mutator. The broad spectrum and high upper limit of the frequency of occurrence suggested ubiquitous roles for UV radiation in accelerating the evolutionary process.

SUBMITTER: Shibai A

PROVIDER: S-EPMC5674018 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Mutation accumulation under UV radiation in Escherichia coli.

Shibai Atsushi A Takahashi Yusuke Y Ishizawa Yuka Y Motooka Daisuke D Nakamura Shota S Ying Bei-Wen BW Tsuru Saburo S

Scientific reports 20171106 1

Mutations are induced by not only intrinsic factors such as inherent molecular errors but also by extrinsic mutagenic factors such as UV radiation. Therefore, identifying the mutational properties for both factors is necessary to achieve a comprehensive understanding of evolutionary processes both in nature and in artificial situations. Although there have been extensive studies on intrinsic factors, the mutational profiles of extrinsic factors are poorly understood on a genomic scale. Here, we ...[more]

PMID: 29109412

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Project description:The DinB (PolIV) protein of Escherichia coli participates in several cellular functions. We investigated a dinB mutation, ?(dinB-yafN)883(::kan) [referred to as ?dinB883], which strongly sensitized E. coli cells to both UV- and X-radiation killing. Earlier reports indicated dinB mutations had no obvious effect on UV radiation sensitivity which we confirmed by showing that normal UV radiation sensitivity is conferred by the ?dinB749 allele. Compared to a wild-type strain, the ?dinB883 mutant was most sensitive (160-fold) in early to mid-logarithmic growth phase and much less sensitive (twofold) in late log or stationary phases, thus showing a growth phase-dependence for UV radiation sensitivity. This sensitizing effect of ?dinB883 is assumed to be completely dependent upon the presence of UmuDC protein; since the ?dinB883 mutation did not sensitize the ?umuDC strain to UV radiation killing throughout log phase and early stationary phase growth. The DNA damage checkpoint activity of UmuDC was clearly affected by ?dinB883 as shown by testing a umuC104 ?dinB883 double-mutant. The sensitivities of the ?umuDC strain and the ?dinB883 ?umuDC double-mutant strain were significantly greater than for the ?dinB883 strain, suggesting that the ?dinB883 allele only partially suppresses UmuDC activity. The ?dinB883 mutation partially sensitized (fivefold) uvrA and uvrB strains to UV radiation, but did not sensitize a ?recA strain. A comparison of the DNA sequences of the ?dinB883 allele with the sequences of the ?(dinB-yafN)882(::kan) and ?dinB749 alleles, which do not sensitize cells to UV radiation, revealed ?dinB883 is likely a "gain-of-function" mutation. The ?dinB883 allele encodes the first 54 amino acids of wild-type DinB followed by 29 predicted residues resulting from the continuation of the dinB reading frame into an adjacent insertion fragment. The resulting polypeptide is proposed to interfere directly or indirectly with UmuDC function(s) involved in protecting cells against the lethal effects of radiation.

Dataset Information

Mutation accumulation under UV radiation in Escherichia coli.

Publications

Mutation accumulation under UV radiation in Escherichia coli.

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