Passage-dependent accumulation of somatic mutations in mesenchymal stromal cells during in vitro culture revealed by whole genome sequencing.
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ABSTRACT:
SUBMITTER: Kim M
PROVIDER: S-EPMC5674020 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Publications
Passage-dependent accumulation of somatic mutations in mesenchymal stromal cells during in vitro culture revealed by whole genome sequencing.
Kim Myungshin M Rhee Je-Keun JK Choi Hayoung H Kwon Ahlm A Kim Jiyeon J Lee Gun Dong GD Jekarl Dong Wook DW Lee Seungok S Kim Yonggoo Y Kim Tae-Min TM
Scientific reports 20171106 1
Human mesenchymal stromal cells (MSCs) have served as a major cellular resource for cell-based immunomodulatory and regenerative therapies. However, genomic instability may accumulate during ex vivo expansion of MSCs, thereby increasing the potential of malignant transformation. Here, we performed whole genome sequencing of two peripheral blood-derived MSC lines (MSC1 and MSC2) at various passages (passage 1 [P1] to P9). The majority of single-nucleotide variations (SNVs) occurred in later passa ...[more]
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