Dataset Information
Nanofiber-based sutures induce endogenous antimicrobial peptide.
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ABSTRACT: Aim
The aim of this study was to develop nanofiber-based sutures capable of inducing endogenous antimicrobial peptide production.Methods
We used co-axial electrospinning deposition and rolling to fabricate sutures containing pam3CSK4 peptide and 25-hydroxyvitamin D3 (25D3).Results
The diameters and mechanical properties of the sutures were adjustable to meet the criteria of United States Pharmacopeia designation. 25D3 exhibited a sustained release from nanofiber sutures over 4 weeks. Pam3CSK4 peptide also showed an initial burst followed by a sustained release over 4 weeks. The co-delivery of 25D3 and pam3CSK4 peptide enhanced cathelicidin antimicrobial peptide production from U937 cells and keratinocytes compared with 25D3 delivery alone. In addition, the 25D3/pam3CSK4 peptide co-loaded nanofiber sutures did not significantly influence proliferation of keratinocytes, fibroblasts, or the monocytic cell lines U937 and HL-60.Conclusion
The use of 25D3/pam3CSK4 peptide co-loaded nanofiber sutures could potentially induce endogenous antimicrobial peptide production and reduce surgical site infections.
SUBMITTER: Chen S
PROVIDER: S-EPMC5674207 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Publications
Nanofiber-based sutures induce endogenous antimicrobial peptide.
Nanomedicine (London, England) 20170929 21
<h4>Aim</h4>The aim of this study was to develop nanofiber-based sutures capable of inducing endogenous antimicrobial peptide production.<h4>Methods</h4>We used co-axial electrospinning deposition and rolling to fabricate sutures containing pam3CSK4 peptide and 25-hydroxyvitamin D<sub>3</sub> (25D<sub>3</sub>).<h4>Results</h4>The diameters and mechanical properties of the sutures were adjustable to meet the criteria of United States Pharmacopeia designation. 25D<sub>3</sub> exhibited a sustained ...[more]
