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Poly(N-isopropylacrylamide) modified polydopamine as a temperature-responsive surface for cultivation and harvest of mesenchymal stem cells.

ABSTRACT: A thermo-responsive surface was fabricated by depositing poly(N-isopropylacrylamide) (PNIPAAm) onto polydopamine coated cell culture substrata through free radical polymerization for the purpose of culturing and harvesting human mesenchymal stem cells (hMSCs). Human MSCs were cultured onto the PNIPAAm-g-polydopamine coated surface and harvested by changing from physiological to ambient temperature. The produced PNIPAAm-g-polydopamine surface was characterized by atomic force microscopy, Fourier transform infrared spectroscopy, nuclear magnetic resonance, water contact angle measurement, differential scanning calorimetry, and cell culture studies. Our results revealed that hMSCs could be detached from the PNIPAAm-g-polydopamine surface within 60 min after switching the temperature from 37 °C to room temperature. The detached hMSCs were able to proliferate on the PNIPAAm-g-polydopamine coated surface for further growth and harvest.

SUBMITTER: Zhang J

PROVIDER: S-EPMC5674794 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Poly(N-isopropylacrylamide) modified polydopamine as a temperature-responsive surface for cultivation and harvest of mesenchymal stem cells.

Zhang Jun J Peng Ching-An CA

Biomaterials science 20171001 11

A thermo-responsive surface was fabricated by depositing poly(N-isopropylacrylamide) (PNIPAAm) onto polydopamine coated cell culture substrata through free radical polymerization for the purpose of culturing and harvesting human mesenchymal stem cells (hMSCs). Human MSCs were cultured onto the PNIPAAm-g-polydopamine coated surface and harvested by changing from physiological to ambient temperature. The produced PNIPAAm-g-polydopamine surface was characterized by atomic force microscopy, Fourier ...[more]

PMID: 29022603

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Project description:In this paper, we report detailed experimental observations of unusual changes in the thickness of solid poly(N-isopropylacrylamide) (PNIPAM) ultrathin films, which are well known to have temperature-responsive hydrophilic-hydrophobic switching properties. To date, a number of studies have been carried out on the bulk and the brush forms of PNIPAM in contact with liquid water, as well as in highly humid environments, and, recently, these ultrathin films have been preliminarily shown to exhibit temperature responses even under low-humidity, ambient conditions. In this work, the thicknesses of ultrathin PNIPAM films in a temperature/moisture-controlled sample stage were monitored continuously using multichannel X-ray reflectometry. At room temperature, the sample thickness showed an unexpected increase after thermal treatment at 70 °C for 3 h. In the temperature cycle between 15 and 60 °C, heating and cooling resulted in some clear differences. During cooling, initially, the thickness was almost constant but began to increase when the temperature exceeded 33 °C, which corresponds to the lower critical solution temperature (LCST). This observation indicates that the PNIPAM ultrathin film is sensitive to the small amounts of water contained in the air, even under ambient, low-humidity conditions. On the other hand, during heating run from 15 to 60 °C, the humidity dependence was monotonic, and no specific changes in the PNIPAM films were observed at around the LCST. By studying the humidity dependence, we found that the hydrophilic and hydrophobic states of the PNIPAM ultrathin film exhibit different temperature dependence behaviors. In addition, we found that swelling takes place even under low-moisture conditions. To understand the difference in the thickness changes observed on cooling and heating further, some models considering the effect of the boundary conditions in the polymer ultrathin film system were considered. In the case of the ultrathin film, the hydrophilic/hydrophobic switching property occurred only in the surface layer, which dominated the absorption of water molecules from air. In contrast, the interface layer was time-stable and provided an escape route for water molecules during heating.

Dataset Information

Poly(N-isopropylacrylamide) modified polydopamine as a temperature-responsive surface for cultivation and harvest of mesenchymal stem cells.

Publications

Poly(N-isopropylacrylamide) modified polydopamine as a temperature-responsive surface for cultivation and harvest of mesenchymal stem cells.

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