Ontology highlight
ABSTRACT:
SUBMITTER: Gala R
PROVIDER: S-EPMC5675596 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Gala Rohan R Lebrecht Daniel D Sahlender Daniela A DA Jorstad Anne A Knott Graham G Holtmaat Anthony A Stepanyants Armen A
eLife 20171023
The ability to measure minute structural changes in neural circuits is essential for long-term in vivo imaging studies. Here, we propose a methodology for detection and measurement of structural changes in axonal boutons imaged with time-lapse two-photon laser scanning microscopy (2PLSM). Correlative 2PLSM and 3D electron microscopy (EM) analysis, performed in mouse barrel cortex, showed that the proposed method has low fractions of false positive/negative bouton detections (2/0 out of 18), and ...[more]