Cellular and exosome mediated molecular defense mechanism in bovine granulosa cells exposed to oxidative stress.

ABSTRACT: Various environmental insults including diseases, heat and oxidative stress could lead to abnormal growth, functions and apoptosis in granulosa cells during ovarian follicle growth and oocyte maturation. Despite the fact that cells exposed to oxidative stress are responding transcriptionally, the potential release of transcripts associated with oxidative stress response into extracellular space through exosomes is not yet determined. Therefore, here we aimed to investigate the effect of oxidative stress in bovine granulosa cells in vitro on the cellular and exosome mediated defense mechanisms. Bovine granulosa cells were aspirated from ovarian follicles and cultured in DMEM/F-12 Ham culture medium supplemented with 10% exosome-depleted fetal bovine serum. In the first experiment sub-conflu

SUBMITTER: Saeed-Zidane M 

PROVIDER: S-EPMC5678720 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature