Dataset Information

SIDT2 Transports Extracellular dsRNA into the Cytoplasm for Innate Immune Recognition.

ABSTRACT: Double-stranded RNA (dsRNA) is a common by-product of viral infections and acts as a potent trigger of antiviral immunity. In the nematode C. elegans, sid-1 encodes a dsRNA transporter that is highly conserved throughout animal evolution, but the physiological role of SID-1 and its orthologs remains unclear. Here, we show that the mammalian SID-1 ortholog, SIDT2, is required to transport internalized extracellular dsRNA from endocytic compartments into the cytoplasm for immune activation. Sidt2-deficient mice exposed to extracellular dsRNA, encephalomyocarditis virus (EMCV), and herpes simplex virus 1 (HSV-1) show impaired production of antiviral cytokines and-in the case of EMCV and HSV-1-reduced survival. Thus, SIDT2 has retained the dsRNA transport activity of its C. elegans ortholog, and this transport is important for antiviral immunity.

SUBMITTER: Nguyen TA

PROVIDER: S-EPMC5679266 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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SIDT2 Transports Extracellular dsRNA into the Cytoplasm for Innate Immune Recognition.

Nguyen Tan A TA Smith Blake R C BRC Tate Michelle D MD Belz Gabrielle T GT Barrios Marilou H MH Elgass Kirstin D KD Weisman Alexandra S AS Baker Paul J PJ Preston Simon P SP Whitehead Lachlan L Garnham Alexandra A Lundie Rachel J RJ Smyth Gordon K GK Pellegrini Marc M O'Keeffe Meredith M Wicks Ian P IP Masters Seth L SL Hunter Craig P CP Pang Ken C KC

Immunity 20170912 3

Double-stranded RNA (dsRNA) is a common by-product of viral infections and acts as a potent trigger of antiviral immunity. In the nematode C. elegans, sid-1 encodes a dsRNA transporter that is highly conserved throughout animal evolution, but the physiological role of SID-1 and its orthologs remains unclear. Here, we show that the mammalian SID-1 ortholog, SIDT2, is required to transport internalized extracellular dsRNA from endocytic compartments into the cytoplasm for immune activation. Sidt2- ...[more]

PMID: 28916264

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Dataset Information

SIDT2 Transports Extracellular dsRNA into the Cytoplasm for Innate Immune Recognition.

Publications

SIDT2 Transports Extracellular dsRNA into the Cytoplasm for Innate Immune Recognition.

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