Ontology highlight
ABSTRACT:
SUBMITTER: Knafo S
PROVIDER: S-EPMC5681248 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Knafo Steven S Prendergast Andrew A Thouvenin Olivier O Figueiredo Sophie Nunes SN Wyart Claire C
Bio-protocol 20170901 18
The proof of concept for bioluminescence monitoring of neural activity in zebrafish with the genetically encoded calcium indicator <i>GFP-aequorin</i> has been previously described (Naumann <i>et al</i>., 2010) but challenges remain. First, bioluminescence signals originating from a single muscle fiber can constitute a major pitfall. Second, bioluminescence signals emanating from neurons only are very small. To improve signals while verifying specificity, we provide an optimized 4 steps protocol ...[more]