Unknown

Dataset Information

0

Characterization of Pseudomonas spp. and Associated Proteolytic Properties in Raw Milk Stored at Low Temperatures.

ABSTRACT: Milk spoilage is caused by the presence of proteolytic enzymes produced by Pseudomonas spp. during storage at low temperatures. The aim of this study was to identify Pseudomonas spp. in raw milk and investigate their associated proteolytic properties at low temperatures. Raw milk samples (n = 87) were collected from 87 bulk tanks in Shaanxi Province in China. Pseudomonas spp. were identified using Pseudomonas specific 16S, universal 16S rRNA sequencing, and rpoB gene sequencing. The proteolytic properties of Pseudomonas spp. were examined using milk agar, quantitative trinitrobenzenesulfonic acid assay, and by the presence of alkaline metallopeptidase gene (aprX). A total 143 isolates from all 87 samples were confirmed as Pseudomonas, and were identified as belonging to 14 Pseudomonas species. Of these, 40 (28.0%) isolates revealed proteolysis on milk agar at 2°C, 74 (51.8%) at 4°C, 104 (72.7%) at 7°C, and 102 (71.3%) at 10°C. However, proteolytic activity of 45 (31.5%) isolates exceeded 2 ?mol of glycine equivalents per mL at 7°C, followed by 43 (30.1%) at 10°C, 18 (12.6%) at 4°C, and 7 (4.9%) at 2°C. The results reveal proteolytic activity of Pseudomonas spp. present in milk and their spoilage potential at different temperatures.

SUBMITTER: Meng L 

PROVIDER: S-EPMC5682325 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Characterization of <i>Pseudomonas</i> spp. and Associated Proteolytic Properties in Raw Milk Stored at Low Temperatures.

Meng Lu L   Zhang Yangdong Y   Liu Huimin H   Zhao Shengguo S   Wang Jiaqi J   Zheng Nan N  

Frontiers in microbiology 20171108

Milk spoilage is caused by the presence of proteolytic enzymes produced by <i>Pseudomonas</i> spp. during storage at low temperatures. The aim of this study was to identify <i>Pseudomonas</i> spp. in raw milk and investigate their associated proteolytic properties at low temperatures. Raw milk samples (<i>n</i> = 87) were collected from 87 bulk tanks in Shaanxi Province in China. <i>Pseudomonas</i> spp. were identified using <i>Pseudomonas</i> specific 16S, universal <i>16S rRNA</i> sequencing,  ...[more]

Similar Datasets

Unknown The Ability of Shiga Toxin-Producing <i>Escherichia coli</i> to Grow in Raw Cow's Milk Stored at Low Temperatures.
| S-EPMC9656703 | biostudies-literature
Unknown Identification, proteolytic activity quantification and biofilm-forming characterization of Gram-positive, proteolytic, psychrotrophic bacteria isolated from cold raw milk.
| S-EPMC10499245 | biostudies-literature
Unknown Molecular and phenotypic characterization of Pseudomonas spp. isolated from milk.
| S-EPMC101459 | biostudies-literature
Unknown Characterization of Milkisin, a Novel Lipopeptide With Antimicrobial Properties Produced By Pseudomonas sp. UCMA 17988 Isolated From Bovine Raw Milk.
| S-EPMC5985324 | biostudies-literature
Unknown Antibiotic Resistance Patterns of <i>Pseudomonas</i> spp. Isolated From Raw Milk Revealed by Whole Genome Sequencing.
| S-EPMC7326020 | biostudies-literature
Unknown Identification of the Enterotoxigenic Potential of <i>Staphylococcus</i> spp. from Raw Milk and Raw Milk Cheeses.
| S-EPMC10819113 | biostudies-literature
Unknown Milk-deteriorating exoenzymes from Pseudomonas fluorescens 041 isolated from refrigerated raw milk.
| S-EPMC4512081 | biostudies-literature
Unknown <i>Pseudomonas koreensis</i> Recovered From Raw Yak Milk Synthesizes a β-Carboline Derivative With Antimicrobial Properties.
| S-EPMC6681700 | biostudies-literature
Unknown Culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits.
| S-EPMC2168224 | biostudies-literature
Unknown Isolation and characterization of Corynebacterium spp. from bulk tank raw cow's milk of different dairy farms in Germany.
| S-EPMC5884509 | biostudies-literature