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Nitric oxide is critical for avoiding hepatic lipid overloading via IL-6 induction during liver regeneration after partial hepatectomy in mice.


ABSTRACT: Nitric oxide (NO), generated from L-arginine by three different isoforms of nitric oxide synthase (NOS), is a pleiotropic factor to regulate physiological functions in almost every organ and tissue. Each knockout mouse of iNOS or eNOS has been used to suggest that NO has a crucial role in liver regeneration after partial hepatectomy (PH), for NO may inhibit caspase 3 activity and is required for EGFR signaling. In previous reports, defective mitochondrial ?-oxidation was observed in eNOS KO mice, and hepatic steatosis was often correlated to deficient liver regeneration, so we focused on metabolic perspective and hypothesized that NO depletion in PH mice would affect hepatocytic lipolysis and impair hepatocytes proliferation. We inhibited all NOS isoforms by administrating L-NG-nitroarginine methyl ester (L-NAME) to PH mice, and hepatocyte DNA synthesis was severely inhibited at 40-44 h post PH in L-NAME (+) group. IL-6 was robustly secreted into circulating blood in L-NAME (-) group, but not in L-NAME (+) group. Down-regulation of carnitine palmytoyltransferase 1A, massive lipid accumulation and elevated endoplasmic reticulum (ER) stress relative genes expression level were observed in L-NAME (+) group mouse liver. The expression level of C/EBP homologous protein, a mediator of ER stress induced apoptosis, significantly increased in L-NAME (+) group. Our findings suggest the lack of NO affected IL-6 induction and hepatocyte lipolysis after PH, consequently leading to excessive hepatic lipid accumulation, elevated ER stress and impaired hepatocyte proliferation.

SUBMITTER: Yu Y 

PROVIDER: S-EPMC5682341 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Nitric oxide is critical for avoiding hepatic lipid overloading via IL-6 induction during liver regeneration after partial hepatectomy in mice.

Yu Yue Y   Tamai Miho M   Tagawa Yoh-Ichi YI  

Experimental animals 20170518 4


Nitric oxide (NO), generated from L-arginine by three different isoforms of nitric oxide synthase (NOS), is a pleiotropic factor to regulate physiological functions in almost every organ and tissue. Each knockout mouse of iNOS or eNOS has been used to suggest that NO has a crucial role in liver regeneration after partial hepatectomy (PH), for NO may inhibit caspase 3 activity and is required for EGFR signaling. In previous reports, defective mitochondrial β-oxidation was observed in eNOS KO mice  ...[more]

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