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Development of a reverse genetics system to generate recombinant Marburg virus derived from a bat isolate.


ABSTRACT: Recent investigations have shown the Egyptian fruit bat (Rousettus aegyptiacus) to be a natural reservoir for marburgviruses. To better understand the life cycle of these viruses in the natural host, a new reverse genetics system was developed for the reliable rescue of a Marburg virus (MARV) originally isolated directly from a R. aegyptiacus bat (371Bat). To develop this system, the exact terminal sequences were first determined by 5' and 3' RACE, followed by the cloning of viral proteins NP, VP35, VP30 and L into expression plasmids. Novel conditions were then developed to efficiently replicate virus mini-genomes followed by the construction of full-length genomic clones from which recombinant wild type and GFP-containing MARVs were rescued. Surprisingly, when these recombinant MARVs were propagated in primary human macrophages, a dramatic difference was found in their ability to grow and to elicit anti-viral cytokine responses.

SUBMITTER: Albarino CG 

PROVIDER: S-EPMC5683708 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Development of a reverse genetics system to generate recombinant Marburg virus derived from a bat isolate.

Albariño César G CG   Uebelhoer Luke S LS   Vincent Joel P JP   Khristova Marina L ML   Chakrabarti Ayan K AK   McElroy Anita A   Nichol Stuart T ST   Towner Jonathan S JS  

Virology 20130905 1-2


Recent investigations have shown the Egyptian fruit bat (Rousettus aegyptiacus) to be a natural reservoir for marburgviruses. To better understand the life cycle of these viruses in the natural host, a new reverse genetics system was developed for the reliable rescue of a Marburg virus (MARV) originally isolated directly from a R. aegyptiacus bat (371Bat). To develop this system, the exact terminal sequences were first determined by 5' and 3' RACE, followed by the cloning of viral proteins NP, V  ...[more]

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