New Transcriptional Reporters to Quantify and Monitor PPAR? Activity.
Ontology highlight
ABSTRACT:
SUBMITTER: Degrelle SA
PROVIDER: S-EPMC5684601 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Publications
New Transcriptional Reporters to Quantify and Monitor PPAR<i>γ</i> Activity.
Degrelle Séverine A SA Shoaito Hussein H Fournier Thierry T
PPAR research 20171101
The peroxisome-proliferator-activated-receptor-<i>γ</i> (PPAR<i>γ</i>) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPAR<i>γ</i>, we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPAR<i>γ</i> activity ...[more]
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