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CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry.

ABSTRACT: Store-operated Ca2+ entry is mediated by Ca2+ release-activated Ca2+ (CRAC) channels following Ca2+ release from intracellular stores. We performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that inhibit store-operated Ca2+ influx. A secondary patch-clamp screen identified CRACM1 and CRACM2 (CRAC modulators 1 and 2) as modulators of Drosophila CRAC currents. We characterized the human ortholog of CRACM1, a plasma membrane-resident protein encoded by gene FLJ14466. Although overexpression of CRACM1 did not affect CRAC currents, RNAi-mediated knockdown disrupted its activation. CRACM1 could be the CRAC channel itself, a subunit of it, or a component of the CRAC signaling machinery.

SUBMITTER: Vig M 

PROVIDER: S-EPMC5685805 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature

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CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry.

Vig M M   Peinelt C C   Beck A A   Koomoa D L DL   Rabah D D   Koblan-Huberson M M   Kraft S S   Turner H H   Fleig A A   Penner R R   Kinet J-P JP  

Science (New York, N.Y.) 20060427 5777

Store-operated Ca2+ entry is mediated by Ca2+ release-activated Ca2+ (CRAC) channels following Ca2+ release from intracellular stores. We performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that inhibit store-operated Ca2+ influx. A secondary patch-clamp screen identified CRACM1 and CRACM2 (CRAC modulators 1 and 2) as modulators of Drosophila CRAC currents. We characterized the human ortholog of CRACM1, a plasma membrane-resident protein encoded by gen  ...[more]

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