Dataset Information

Optimization of extraction process of inulin from Indian millets (jowar, bajra and ragi)-characterization and cost analysis.

ABSTRACT: Prebiotic biomolecule, namely, inulin was extracted from Indian millets, namely, jowar (Sorghum vulgare), bajra (Pennisetum glaucum) and ragi (Eleusine coracana). Through qualitative assessment using Fourier Transform Infrared spectroscopy, the presence of functional groups of inulin in the above mentioned Indian millets were verified. The values of degree of polymerization of inulin derived from jowar, bajra and ragi were determined to be 27, 39 and 23 respectively. A comparative analysis of growth of Lactobacillus casei was carried out in presence of both lactose and inulin extracted from three millets and the commercial one. It was observed that the bajra inulin and lactose combination exhibited the best bacterial growth. The prebiotic effectiveness of different varieties of inulin was calculated to be in the following order: bajra > jowar > ragi > commercial inulin. Therefore the results on bajra inulin were highlighted in this article. Inulin yield from bajra was optimized as a function of temperature, HCl concentration and heating period. The maximum inulin yield (0.4727 g/g bajra) was obtained at temperature 70 °C, HCl concentration of 0.8 M and heating period of 60 min. The prebiotic activity score of bajra inulin (= 3.2) was measured to be much higher than commercial inulin (= 1.0). Growth dynamics of Lactobacillus casei on lactose, bajra inulin and mixture of lactose and bajra inulin were found to be of Monod type, Haldane type and Multi-substrate-summative type respectively. The techno-economic analysis based on the production cost of inulin from raw bajra seeds suggested that it was much cheaper than commercial inulin.

SUBMITTER: Banerjee D

PROVIDER: S-EPMC5686010 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Optimization of extraction process of inulin from Indian millets (jowar, bajra and ragi)-characterization and cost analysis.

Banerjee Debolina D Chowdhury Ranjana R Bhattacharya Pinaki P

Journal of food science and technology 20171010 13

Prebiotic biomolecule, namely, inulin was extracted from Indian millets, namely, jowar (Sorghum vulgare), bajra (Pennisetum glaucum) and ragi (Eleusine coracana). Through qualitative assessment using Fourier Transform Infrared spectroscopy, the presence of functional groups of inulin in the above mentioned Indian millets were verified. The values of degree of polymerization of inulin derived from jowar, bajra and ragi were determined to be 27, 39 and 23 respectively. A compa ...[more]

PMID: 29184236

Similar Datasets

Project description:PurposeAralia taibaiensis, a medicinal food plant, and total saponins from its root bark extract inhibit α-glucosidase activity, which is associated with type 2 diabetes; however, the inhibitory mechanism is unknown. Furthermore, a green extraction technique superior to conventional hot reflux extraction (HRE) is needed for the rapid and easy extraction of A. taibaiensis total saponins (TSAT) to exploit and utilize this resource. Our aim was to develop a green extraction method for obtaining TSAT and to investigate the mechanism by which TSAT inhibits α-glucosidase.Materials and methodsIn this study, the ultrasound-assisted extraction (UAE) process was optimized using a Box-Behnken design, and the extraction mechanism was investigated using scanning electron microscopy (SEM). High-performance liquid chromatography (HPLC) was used for qualitative and quantitative analyses of TSAT. In vitro glycosylation assays, enzyme kinetics, fluorescence spectroscopy measurements, atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FT-IR) and molecular docking techniques were used to investigate the mechanism by which the A. taibaiensis active ingredients inhibit α-glucosidase.ResultsThe optimal parameters for the extraction yield were obtained as an ethanol concentration of 73%, ultrasound time of 34 min, ultrasound temperature of 61 °C and solid-liquid ratio of 16 g/mL, which were better than HRE. The SEM analysis showed that UAE effectively disrupted plant cells, thus increasing the TSAT yield. In vitro α-glucosidase inhibition experiments showed that both TSAT and its active ingredient, araloside A, inhibited α-glucosidase activity by binding to α-glucosidase, thereby changing the conformation and microenvironment of α-glucosidase to subsequently inhibit enzyme activity.ConclusionThe optimal extraction conditions identified here established a basis for future scale-up of ultrasound extraction parameters with the potential for obtaining maximum yields. In vitro enzyme inhibition experiments investigated the mechanism of the TSAT interaction with α-glucosidase and further explored whether araloside A may be the main contributor to the good inhibition of α-glucosidase activity by TSAT.

Dataset Information

Optimization of extraction process of inulin from Indian millets (jowar, bajra and ragi)-characterization and cost analysis.

Publications

Optimization of extraction process of inulin from Indian millets (jowar, bajra and ragi)-characterization and cost analysis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets