Systematic analysis of Ca²⁺ homeostasis in Saccharomyces cerevisiae based on chemical-genetic interaction profiles.

ABSTRACT: We investigated the global landscape of Ca²⁺ homeostasis in budding yeast based on high-dimensional chemical-genetic interaction profiles. The morphological responses of 62 Ca²⁺-sensitive (cls) mutants were quantitatively analyzed with the image processing program CalMorph after exposure to a high concentration of Ca²⁺ After a generalized linear model was applied, an analysis of covariance model was used to detect significant Ca²⁺-cls interactions. We found that high-dimensional, morphological Ca²⁺-cls interactions were mixed with positive (86%) and negative (14%) chemical-genetic interactions, whereas one-dimensional fitness Ca²⁺-cls interactions were all negative in principle. Clustering analysi

SUBMITTER: Ghanegolmohammadi F 

PROVIDER: S-EPMC5687040 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature